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KMID : 1094720070120040366
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 4 p.366 ~ p.371
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Biothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetry
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Kim Ki-Hyung
Lee Eun-Kyu
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Abstract
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In this study, we used ITC (isothermal titration calorimetry) to quantitatively investigate the impacts of temperature and protein concentration on adsorption behavior on a solid surface, using BSA (bovine serum albumin) as a model protein, and alum (aluminum hydroxide) gel as an adsorbent. The zeta potential measurement for alum gel (0.25 mV at pH 9.3) revealed that its surface charge was not strong enough for electrostatic interaction. ITC analysis showed that the BSA-alum gel interaction was entropy-driven, suggesting that during adsorption, water molecules were expelled from the hydration layers of the alum gel and BSA. Therefore, the major mechanism for the BSA-alum gel interaction was hydrophobic interaction rather than electrostatic interaction. This biothermodynamic approach can be helpful not only to identify interaction mechanisms, but also to explore the optimum conditions for protein-adsorbent interactions.
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KEYWORD
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ITC, alum gel, adjuvant, adsorption, biothermodynamics, heat of adsorption
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